What is the reason for using 0.5m sucrose solution in the chloroplast isolation?

any one have an idea





help please . What is the reason for using 0.5m sucrose solution in the chloroplast isolation?
i guess using 0.5 molar solution has the correct number of sucrose particles per cubic metre to be able to sustain chloroplast function. What is the reason for using 0.5m sucrose solution in the chloroplast isolation?
is a isotonic solution for chloroplasts. if You use tap water, se revienta (in spanish)

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