One possibility could be that they consider themselves to be 'not part' of the people they travel among and keep their own council which attitude will invariably alienate them from the most part of their fellow man.Travellers have been distrusted ever since man first began to settle as they were once a serious threat to life and foodstocks and in many places around the world bands of wanderers still are a great danger to settlements and travelling individuals.As people who live in settled communities shun the travellers so the travelling folk keep their own beliefs and culture wrapped around tightly,a form of uniform and badge.What do you think is the reason for the cultural isolation of Irish travellers and Romany gypsies?
They are very private people and that's the way they like it. Because of that people that don't know or understand mistrust them and make up horrible stories about them. The real Irish Travellers and Romany Gypsies are very honest and clean its the New age travellers and the like that give them a bad nameWhat do you think is the reason for the cultural isolation of Irish travellers and Romany gypsies?
My mom is Romani. Honestly, people discriminate against us. We don't know why. We haven't done anything to deserve being treated the way we do. We get denied citizenship, rights of the countries we live in and mention you are a gypsy, you're branded for life. It'd be like walking around screaming ';I'm a Jew!'; to German soldiers during the Holocaust. We don't want to deal with outsiders because of the Europeans, whom we've lived around for over a thousand years, have a problem with us. We don't want to deal with the problem of being denied basic human rights over and over so we keep to ourselves. My dad is Greek and I'm the only ';half-Romani'; in a family of over 200 and I've never met another half Romani and that's saying something. My Greek family, they can't stand me. I don't know why. I'm not a barbarian. For example, an estimated number of no less than 200,000 Romani people were killed during the Holocaust (some estimate as high as 800,000) by the Germans but Holocaust memorial groups won't recognize us or give us any recognition for what our people went through. We just don't count, it seems. It's not so much that we want to be isolated. It's just that we have to. Romani people get killed for being Romani everyday and no one investigates or gets charged in the murders because deep down, the cops, the judges and the majority population in general think ';good riddance.'; They just had an Anti-Romani march in Czech Republic a few months ago.
Because they want it that way..they have been taught over the years to stay amongst themselves as much as possible so the habit 'sticks' /gets passed down
Friday, January 8, 2010
What companies manufacture class I-division I fire rated isolation valves?
Class I Division I implies that it is in an environment that frequently has combustible material in the vicinity. If you are aware of any companies that manufacture such valves, I will call them up and do the rest of the legwork to see if they can fulfill our needs.What companies manufacture class I-division I fire rated isolation valves?
To answer the question better depends on what type of valve is required.
A few manufacturers that come to mind are, DeZurik, Apollo, Fisher (Emerson process), Flowserve and Asco.What companies manufacture class I-division I fire rated isolation valves?
You seem to be asking about NEC Class 1 Div 1. If so, are you asking about the devices that control the valve or the sealing portion of the valve?
The type of valve does matter because control valve manufacturers like Fisher can produce valves that are complete and can meet Class 1 Div 1 requirements. Some other valve manufacturers do not make valves complete with actuators or other controls. These features are added by others.
Control valves such as Fisher are not fire rated.
The use of Class 1 Div 1 to address the fluid containing ability of any valve simply isn't done. You can get valves that meet API and other ratings similar to the ball valves at the site below.
To answer the question better depends on what type of valve is required.
A few manufacturers that come to mind are, DeZurik, Apollo, Fisher (Emerson process), Flowserve and Asco.What companies manufacture class I-division I fire rated isolation valves?
You seem to be asking about NEC Class 1 Div 1. If so, are you asking about the devices that control the valve or the sealing portion of the valve?
The type of valve does matter because control valve manufacturers like Fisher can produce valves that are complete and can meet Class 1 Div 1 requirements. Some other valve manufacturers do not make valves complete with actuators or other controls. These features are added by others.
Control valves such as Fisher are not fire rated.
The use of Class 1 Div 1 to address the fluid containing ability of any valve simply isn't done. You can get valves that meet API and other ratings similar to the ball valves at the site below.
How do i know when the time is right to put my pregnant mollie in the ';isolation box';?
first i would like to know how i can tell if shes pregnant, shes a mollie and i heard they reproduce fast, she hides alot. so when should i put her in the box so the other fish dont eat the babies?How do i know when the time is right to put my pregnant mollie in the ';isolation box';?
When she looks round and plumish, If she has been with males then yes she is pregnant and they reproduce evry 4-8 weeks, I really dont suggest puting any fish in a breeder/isloation box as it causes MAJOUR stress on the fish, And can and does on some ocasions result in stilll born fry (babies), dead babies and sometimes a dead mother, A mollie is rather large to be put in a breeder box, I only suggest guppies in boxes (well i dont suggest any fish in them.) I suggest you just provide lots of plants (fake or real) and let the babies hide, After all the do reproduce every 1-2 months and you will be over run with babies so just let nature takes its tole and let some babies be eaten.
At the end of the day its up to you if you put the fish in the breeder but put your self in the fishes position, Pregnancy is allready difficult, Never mind puting them in a small 3 x 6in box,
~ GGHow do i know when the time is right to put my pregnant mollie in the ';isolation box';?
I don't bother with such nonsense. My mollies live and thrive and breed in massive numbers in my planted 55g tank. I'm sure a few fry get eaten but thats life. The weak, deformed and stupid ones turn into snacks, the healthy, fast, smart ones stay alive.
When she looks round and plumish, If she has been with males then yes she is pregnant and they reproduce evry 4-8 weeks, I really dont suggest puting any fish in a breeder/isloation box as it causes MAJOUR stress on the fish, And can and does on some ocasions result in stilll born fry (babies), dead babies and sometimes a dead mother, A mollie is rather large to be put in a breeder box, I only suggest guppies in boxes (well i dont suggest any fish in them.) I suggest you just provide lots of plants (fake or real) and let the babies hide, After all the do reproduce every 1-2 months and you will be over run with babies so just let nature takes its tole and let some babies be eaten.
At the end of the day its up to you if you put the fish in the breeder but put your self in the fishes position, Pregnancy is allready difficult, Never mind puting them in a small 3 x 6in box,
~ GGHow do i know when the time is right to put my pregnant mollie in the ';isolation box';?
I don't bother with such nonsense. My mollies live and thrive and breed in massive numbers in my planted 55g tank. I'm sure a few fry get eaten but thats life. The weak, deformed and stupid ones turn into snacks, the healthy, fast, smart ones stay alive.
Which of the following is an example of mechanical isolation?
a. Two species of birds live in the same habitat, one mates in the spring and the other in the summer.
b. The flower structure of one species prevents the transfer of pollen from another species.
c. one species of lizards inhabits the trees, and another species inhabits the ground cover.Which of the following is an example of mechanical isolation?
The answer is b.
Mechanical isolation are isolation because their reproduction organs physically are not compatible to each other, so copulation between them are impossible.Which of the following is an example of mechanical isolation?
B
b. The flower structure of one species prevents the transfer of pollen from another species.
c. one species of lizards inhabits the trees, and another species inhabits the ground cover.Which of the following is an example of mechanical isolation?
The answer is b.
Mechanical isolation are isolation because their reproduction organs physically are not compatible to each other, so copulation between them are impossible.Which of the following is an example of mechanical isolation?
B
How did the isolation policy displayed by the U.S. at WW2 help it 2 start?
And how did it prove that the allies could've done more to stop hitler? thank u, please no balanced arguements.How did the isolation policy displayed by the U.S. at WW2 help it 2 start?
You should focus on when the US got involved with WW2 (1940) versus what was going on in Europe and Asia prior to that.
What effect, if any, the US' earlier entrance into WW2 would have had is hard to say...that quickly starts getting into the realm of speculative fiction.
You should focus on when the US got involved with WW2 (1940) versus what was going on in Europe and Asia prior to that.
What effect, if any, the US' earlier entrance into WW2 would have had is hard to say...that quickly starts getting into the realm of speculative fiction.
Can anyone explain me the procedures involved in isolation of proteins?
please explain me about the column chromatography (that is involved right?) and the function of SDS gel.Can anyone explain me the procedures involved in isolation of proteins?
Proteins can be purified by size or by charge. Gel filtration chromatography on Sulfadex columns separates proteins by size. Ion exchange chromatography on DEAE- or CM-Sulfadex columns separates proteins by their charges. HPLC can also be used. SDS gel electrophoresis is not a separation method because SDS denatures proteins, and is used to check the purity of the final product.Can anyone explain me the procedures involved in isolation of proteins?
Column chromatography *can* be used to isolate proteins, as can SDS PAGE (polyacrylamide gel electrophoresis). These are both forms of chromatography.
Reverse-phase HPLC (High-pressure liquid chromatography) is a method of purifying proteins from a mixture of proteins (and possibly other substances) by their hydrophobicity. The principle is to use a column of packed beads which are hydrophobic in character. You dissolve your protein mixture in an aqueous solution (called the ';immobile phase) and pass it through the column. The proteins will stick to your column, as most proteins are quite hydrophobic.
You then gradually increase the amount of an organic solvent (like acetonitrile) you pass over the column (for example, maybe increasing from 0% to 100% over 1 hour 40 minutes). The different proteins will have different hydrophobic characters: in general, the longer a protein, the more hydrophobic, and the more hydrophobic amino acids it has in it, the more hydrophobic it is. As the percentage of organic solvent (called the ';mobile phase';) increases you will gradually reach a concentration which will start to remove the proteins from the column one at a time - the least hydrophobic will come off first, and the most hydrophobic will come off last.
At the end of the column, you have a detector, which will detect when proteins are coming off by UV absorbance (all proteins absorb UV at a wavelength of 230nm), so you can collect individual fractions which will hopefully contain only one protein.
After this, you can determine which proteins are in each fraction by other methods (like immuonoblotting, or mass spectrometry), and you will then (hopefully) have a pure sample of your protein-of-interest.
There are other methods of column chromatography - like size-exclusion columns, where the largest proteins come through fastest, and the smallest last.
Another refinement uses antibodies against your protein-of-interest, immobilised in the column. You pass your mixture over the column, and only your protein will stick to the antibodies. After running everything through, you wash with a concentrated salt solution, which removes your protein and provides you with a purified sample.
SDS PAGE is another method of separating proteins from a mixture by their size. You dissolve your proteins in a solution containing sodium dodecyl sulphate (SDS) - a detergent which gives the proteins a net negative charge. You then load your mixture into a well at one end of a gel of polyacrylamide.
A current is applied to the gel, so the charged proteins will migrate through the gel; the largest proteins move slowest (as they take longest to pass through the pores in the gel), and the smallest move fastest.
You will usually include a charged dye, which will migrate with the fastest proteins, and when this dye has reached the end of the gel, you stop the current and stain the gel with a protein-detecting stain (like coomassie blue) to show up where all the protein bands are. By comparing with a lane in the gel that contained a set of known-mass standards, you can estimate what weight each of the bands are.
SDS PAGE is not usually used to actually *purify* proteins, but to determine what is in each mixture, as the amounts loaded are usually rather small.
A similar method is used for isolating DNA from a mixture of different-length DNA strands.
A different method, called isoelectric focussing, involves not adding the SDS, loading your samples in the *middle*, and applying an alternating current to the gel (running first one way, then the other). This has the effect of concentrating each protein at a specific location, dependent on its *charge*, rather than its weight.cosmetics makeup
Proteins can be purified by size or by charge. Gel filtration chromatography on Sulfadex columns separates proteins by size. Ion exchange chromatography on DEAE- or CM-Sulfadex columns separates proteins by their charges. HPLC can also be used. SDS gel electrophoresis is not a separation method because SDS denatures proteins, and is used to check the purity of the final product.Can anyone explain me the procedures involved in isolation of proteins?
Column chromatography *can* be used to isolate proteins, as can SDS PAGE (polyacrylamide gel electrophoresis). These are both forms of chromatography.
Reverse-phase HPLC (High-pressure liquid chromatography) is a method of purifying proteins from a mixture of proteins (and possibly other substances) by their hydrophobicity. The principle is to use a column of packed beads which are hydrophobic in character. You dissolve your protein mixture in an aqueous solution (called the ';immobile phase) and pass it through the column. The proteins will stick to your column, as most proteins are quite hydrophobic.
You then gradually increase the amount of an organic solvent (like acetonitrile) you pass over the column (for example, maybe increasing from 0% to 100% over 1 hour 40 minutes). The different proteins will have different hydrophobic characters: in general, the longer a protein, the more hydrophobic, and the more hydrophobic amino acids it has in it, the more hydrophobic it is. As the percentage of organic solvent (called the ';mobile phase';) increases you will gradually reach a concentration which will start to remove the proteins from the column one at a time - the least hydrophobic will come off first, and the most hydrophobic will come off last.
At the end of the column, you have a detector, which will detect when proteins are coming off by UV absorbance (all proteins absorb UV at a wavelength of 230nm), so you can collect individual fractions which will hopefully contain only one protein.
After this, you can determine which proteins are in each fraction by other methods (like immuonoblotting, or mass spectrometry), and you will then (hopefully) have a pure sample of your protein-of-interest.
There are other methods of column chromatography - like size-exclusion columns, where the largest proteins come through fastest, and the smallest last.
Another refinement uses antibodies against your protein-of-interest, immobilised in the column. You pass your mixture over the column, and only your protein will stick to the antibodies. After running everything through, you wash with a concentrated salt solution, which removes your protein and provides you with a purified sample.
SDS PAGE is another method of separating proteins from a mixture by their size. You dissolve your proteins in a solution containing sodium dodecyl sulphate (SDS) - a detergent which gives the proteins a net negative charge. You then load your mixture into a well at one end of a gel of polyacrylamide.
A current is applied to the gel, so the charged proteins will migrate through the gel; the largest proteins move slowest (as they take longest to pass through the pores in the gel), and the smallest move fastest.
You will usually include a charged dye, which will migrate with the fastest proteins, and when this dye has reached the end of the gel, you stop the current and stain the gel with a protein-detecting stain (like coomassie blue) to show up where all the protein bands are. By comparing with a lane in the gel that contained a set of known-mass standards, you can estimate what weight each of the bands are.
SDS PAGE is not usually used to actually *purify* proteins, but to determine what is in each mixture, as the amounts loaded are usually rather small.
A similar method is used for isolating DNA from a mixture of different-length DNA strands.
A different method, called isoelectric focussing, involves not adding the SDS, loading your samples in the *middle*, and applying an alternating current to the gel (running first one way, then the other). This has the effect of concentrating each protein at a specific location, dependent on its *charge*, rather than its weight.
Which Colony Isolation Method should be used on Liquid Culture?
*If you were starting with a liquid culture, which method would you choose to isolate microorganisms and why?
Is the answer to this streak plate because allows isolation of colonies in the 4th quadrant. Or can you not perfrom streak plate technique on liquid cultures. Instead should I put you used spread plate technique then use streak plate technique after the spread plate has been incubated and growth has occured. Then use streak plate on that group?
Your help would be ohhh soo appreciated! Thanks!Which Colony Isolation Method should be used on Liquid Culture?
I THINK YOUR ANSWER IS ON HERE! GOOD LUCK!
http://www.springerlink.com/content/a03d鈥?/a>
Is the answer to this streak plate because allows isolation of colonies in the 4th quadrant. Or can you not perfrom streak plate technique on liquid cultures. Instead should I put you used spread plate technique then use streak plate technique after the spread plate has been incubated and growth has occured. Then use streak plate on that group?
Your help would be ohhh soo appreciated! Thanks!Which Colony Isolation Method should be used on Liquid Culture?
I THINK YOUR ANSWER IS ON HERE! GOOD LUCK!
http://www.springerlink.com/content/a03d鈥?/a>
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