I'm doing a Bio lab and I'm frustrated...I'm assuming it's for cell lysis but why? And why would we incubate the cells ate 56 degrees C for 30 minutes and then 70 degress C for 10 minutes?
HELP!! :) thanks!
Why are cells incubated in a water bath during DNA isolation?
looking at those temeratures you are doing PCR yeah?
the 70 degrees is to denature the DNA. this makes it single stranded instead of the usual double strand. the 56 degrees is for the polymerase to be working at its optimum environment and therefore work best.
the reason it is so much higher than a normal bacterias optimum temp is because it is a polymerase from thermophilus aquatis (taq). this is used because it is a extremophillic bacteria found around underwater vents which are very hot.
it is used so the polymerase itself is not denatured when the DNA is at 70 degrees.
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